F2R Knockout cell line (Huh-7)

F2R Knockout cell line (Huh-7) Order

Catalog#

YKO-H1180

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

F2R Knockout cell line（HuH-7）

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:3

Species

Human

Gene Name

F2R

Gene ID

2149

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Target region

Knockout region: Exon 2 (based on transcript F2R-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HuH-7			STR Info (Cell bank) Cell Line:HuH-7
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	10	11		10	11
D7S820	11	11		11	11
D16S539	10	10		10	10
VWA	16	18		16	18
TH01	7	7		7	7
AMEL	X	X		X	X
TPOX	8	11		8	11
CSF1PO	11	11		11	11
D12S391	20	21
FGA	22	23
D2S1338	19	19
D21S11	30	30
D18S51	15	15
D8S1179	14	14
D3S1358	15	15
D6S1043	13	15
PENTAE	11	11
D19S433	13	14
PENTAD	12	12
D1S1656	16	16

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the F2R gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HuH-7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, F2R gene knockout F2R Knockout cell line（HuH-7） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

F2R Knockout cell line (Huh-7)

Catalog#

YKO-H1180

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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